After a round of linking building block, encode each compound with a given sequence of DNA
Sequence encoded tags (DNA) of the selected hits compounds and obtain their chemical information
Incubate the active immobilized target protein and DNA encoded library for some time; during the time, compounds with strong affinity will bind to the protein while non-binders / weak-binders will be eluted. Denature the protein with heat and elute the selected binders. Elucidate the structure of binders through PCR amplification / DNA sequencing. Re-synthesize off-DNA for confirmation of activity. Optimize chemical structure to generate lead compounds.