OpenDEL™

OpenDEL™ was initially launched by HitGen as a self-service product in 2015 (https://www.nature.com/articles/d43747-020-00040-4). Over years of continuous development and optimization, the platform has evolved to encompass highly diverse small molecule DNA-encoded libraries (DELs) that occupy a broad drug-like chemical space. This enhanced diversity and structural coverage significantly improve the probability of identifying potential hit compounds in a highly efficient and cost-effective manner, making OpenDEL™ a powerful tool for drug discovery and lead optimization.

What is included in OpenDEL^™?

OpenDEL™ provides comprehensive DNA-encoded library (DEL) solutions designed for affinity selection and related research applications. Each package includes the following essential components:

DEL Libraries: Over 3 billion compounds and 50 libraries

PCR Primers: Forward primer (Primer F), Reverse primer (Primer R)

qPCR Standard Curve Sample: For accurate quantification and quality control.

Salmon Sperm DNA (ssDNA):Used as a blocking agent to reduce non-specific binding.

Empty Tubes: For sample preparation and experimental setup.

Standard Selection Protocol: A detailed manual outlining step-by-step procedures for DEL selection experiments.

OpenDEL™ is designed to streamline your drug discovery process, offering all the necessary materials and guidance for efficient and reliable screening.

OpenDEL™ Workflow Overview

1. Target Selection

Researchers design the affinity selection protocol based on their in-depth understanding of the target. HitGen supports this process by providing:

l  A basic screening protocol.

l   Comprehensive DEL information, including reagent details and codon mapping.

l   Technological consultation services to optimize experimental design.

2. Hit Generation

Post-selection samples can be decoded either by HitGen or by the user. If sequencing is performed at HitGen, users will receive:

l   Raw sequencing data.

l   Mapping data with feature intensity for further analysis.

3. Hit Validation

Potential hits identified through data analysis can be validated through the following steps:

l   On-DNA Re-synthesis:Confirm binding ligands directly on the DNA-encoded platform.

l    Off-DNA Re-synthesis: Synthesize compounds for further validation using biochemical, biophysical, or cellular assays.

OpenDEL™ offers a seamless and efficient workflow, from target selection to hit validation, supported by HitGen’s expertise and resources.

Why choose OpenDEL™?

l  Transparent:Full access to DEL information, including reagents and codon mappings.

l  Supportive: Expert technical guidance for selection, sequencing, and analysis.

l  Reliable: High-diversity libraries with robust chemistry and strict QC.

l  Affordable: Cost-effective and accessible for all researchers and flexible collaboration model