Welcome to HitGen

Oligonucleotides

HitGen offers comprehensive solutions for the development of oligonucleotide therapeutics, covering the entire process from research and development to production. 

  1. Oligonucleotides
  2. Biological Evaluation

Biological Evaluation

Precise and Efficient In Vitro Activity Evaluation System
• 
Multi-Model Validation

Compatible with immortalized cell lines, primary cells, and other models, supporting diverse nucleic acid therapeutics including mRNA, siRNA, ASO, Dual-targeting siRNA, and more.

• Multi-Dimensional Analysis

Gene Expression Modulation:

Quantify mRNA knockdown efficiency of target genes via qPCR and RNA-Seq.

Protein Functional Validation:

Accurately detect changes in target protein expression using ELISA, Western Blot, and flow cytometry.




Systematic Druggability Assessment Matrix

Evaluation    Dimension

Technical    Methods

Significance

Stability

Serum/plasma stability   assays, accelerated degradation testing

Optimizing   chemical modification strategies to extend in vivo half-life.

Pharmacokinetics

LC-MS/MS   quantification, tissue distribution analysis

Deciphering   absorption, distribution, and metabolism patterns to guide formulation   design.

Immunogenicity

TLR pathway   activation assays, cytokine release profiling

Mitigating   immunotoxicity risks of nucleic acid drugs.

Cytotoxicity

CCK-8 assays,   LDH release tests, mitochondrial membrane potential detection, Caspase-dependent   apoptosis analysis

Evaluating   safety profiles and screen candidates with high therapeutic indices.

Off-Target   Effects

Whole-transcriptome   sequencing (RNA-Seq), bioinformatics prediction

Filtering   off-target risks and enhance drug specificity.




Differentiated Technical Advantages
• 
High-Sensitivity Detection:

Capable of detecting nucleic acid drug activity as low as 0.1 pM, enabling development for low-abundance targets.
• 
Cross-Species Compatibility:

Covers human, murine, and non-human primate models for seamless preclinical-to-clinical translation.
• 
Customized Services:

Tailor evaluation protocols based on drug mechanisms of action (MOA) and project requirements.

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