Biological Evaluation

Precise and Efficient In Vitro Activity Evaluation System
• Multi-Model Validation

Compatible with immortalized cell lines, primary cells, and other models, supporting diverse nucleic acid therapeutics including mRNA, siRNA, ASO, Dual-targeting siRNA, and more.

• Multi-Dimensional Analysis

Gene Expression Modulation:

Quantify mRNA knockdown efficiency of target genes via qPCR and RNA-Seq.

Protein Functional Validation:

Accurately detect changes in target protein expression using ELISA, Western Blot, and flow cytometry.

Systematic Druggability Assessment Matrix

Evaluation Dimension	Technical Methods	Significance
Stability	Serum/plasma stability assays, accelerated degradation testing	Optimizing chemical modification strategies to extend in vivo half-life.
Pharmacokinetics	LC-MS/MS quantification, tissue distribution analysis	Deciphering absorption, distribution, and metabolism patterns to guide formulation design.
Immunogenicity	TLR pathway activation assays, cytokine release profiling	Mitigating immunotoxicity risks of nucleic acid drugs.
Cytotoxicity	CCK-8 assays, LDH release tests, mitochondrial membrane potential detection, Caspase-dependent apoptosis analysis	Evaluating safety profiles and screen candidates with high therapeutic indices.
Off-Target Effects	Whole-transcriptome sequencing (RNA-Seq), bioinformatics prediction	Filtering off-target risks and enhance drug specificity.

Differentiated Technical Advantages
• High-Sensitivity Detection:

Capable of detecting nucleic acid drug activity as low as 0.1 pM, enabling development for low-abundance targets.
• Cross-Species Compatibility:

Covers human, murine, and non-human primate models for seamless preclinical-to-clinical translation.
• Customized Services:

Tailor evaluation protocols based on drug mechanisms of action (MOA) and project requirements.

Oligonucleotides

Biological Evaluation

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