Welcome to HitGen

About

Enable Innovative Drug Discover with DEL, FBDD and SBDD

  1. 关于我们
  2. Publications

Exploring the Lower Limit of Individual DNA-Encoded Library Molecules in Selection 发布时间:2019-12-20

Abstract:

DNA-encoded library (DEL) technology has been used as an ultra-high-throughput screening approach for hit identification of drug targets. This process is an affinity-based selection and requires incubation of DEL molecules with the target. Currently, in most reported cases, the input (i.e., the copy number) of individual DEL molecules varies from 105 to 107. With the ever-increasing DEL size and screening cost, lowering the input of DEL molecules while maintaining an appropriate signal-to-noise ratio in a selection is of paramount importance. In this article, we varied the input of DEL ranging from 103 to 105 in selections with two different protein targets to explore the lower limit of DEL molecule input. The results could facilitate the optimization of the DEL selection process and reduce costs related to library consumption.


  

SLAS DISCOVERY: Advancing the Science of Drug Discovery. 2020;25(5):523-529. 

DOI:10.1177/2472555219893949

Link:https://journals.sagepub.com/doi/10.1177/2472555219893949

  


Thanks for reading!

For more details on how we can advance your innovative drug discovery projects
验证码
By submitting your information, you acknowledge having received, read and understood our Privacy Notice as made available above.

We use cookies to provide a better web experience.
By using our site, you acknowledge our use of cookies and please read our Cookie Notice for More information