Welcome to HitGen

成都先导

做最具创新力的生物医药企业     用最先进的科学技术赋能创新药研发

  1. 关于我们
  2. 发表文章

A Simple Method for Determining Compound Affinity and Chemical Yield From DNA-encoded Library Selections 发布时间:2020-06-18

摘要:


DNA-encoded libraries (DELs) can contain billions of unique chemical species; selecting against such large inputs, it is typical to find more candidate binders than is reasonable to pursue for follow-up synthesis and testing. Given this wealth of choices, common practice is to limit synthesis to only those compounds estimated to have the greatest chance of being high-affinity binders; of the many potential factors contributing to this estimation, the strength of the selection signal of a candidate binder is always important. We define here methods and equations which relate the theoretical selection signal of a compound to its affinity and chemical yield. Tests using known binders of BRD4 and ROCK2 support the theory backing these equations and suggest they should be of use for prospectively determining affinity and chemical yield from primary DEL selection data.


Biochemical and Biophysical Research Communications Volume 527, Issue 1, 18 June 2020, Pages 250-256

https://doi.org/10.1016/j.bbrc.2020.04.024

Link: https://www.sciencedirect.com/science/article/abs/pii/S0006291X20307488


联系我们

业务咨询与服务
提交此表格即表示我同意成都先导可以按照隐私政策说明中所述的方式收集、处理我的数据。

为了使您在浏览本网站时获得最佳的体验,您需同意我们对Cookies的使用。想要了解更多有关于Cookies的信息,请阅读我们的 隐私政策。