Welcome to HitGen

Capabilities

One-stop Technology Platform to Accelerate Drug Discovery and Development

  1. Capabilities
  2. Protein Engineering, Expression and Purification

Protein Engineering, Expression and Purification

HitGen's protein science team expertise includes detailed target know-how in various target classes, construct design for a variety of available expression systems, multi-protein complex assembly, refolding and purification skills. We focus on novel protein targets and customized Gene-to-Protein projects, whether for crystallization purposes, screening, assay development or general drug discovery research. We produce proteins according to projects’ need and/or our clients’ specifications, starting from a gene construct designed for their purpose, with multiple tag variations and functional labelling (e.g. biotinylation), as well as defined post-translational modifications. 


image.png


Most target constructs are rationally designed by a knowledge-based design process, including experience, literature data, and specific needs for downstream application. The purification and quality control demands are set together with our internal downstream scientists or external customers to meet the project requirements.


Our expression platform includes a full suite of expression systems (E.coli, yeast, insect cells and mammalian cells). The detailed host cell and vector information for the 3 main expression systems, as well as the tag types frequently used are listed below.


1.2.1-Protein Engineering, Expression and Purification-1.png


For difficult targets, we use focused small-scale screens to find suitable boundaries.


We purify all proteins in-house on AKTA FPLC systems using the classic chromatographic methods, such as affinity chromatography, ion exchange and size-exclusion chromatography. All purified proteins can be tested with UV spectro-photometry and SDS page gel analysis to ensure >90% pure. We usually produce the proteins of interest in multi-milligram quantities.


A complete protein science solution is available at HitGen, including:

  • Construct design and molecular cloning

  • Expression system optimization 

  • Purification by affinity, ion-exchange and size-exclusion chromatography

  • Protein characterization


High quality proteins can support and facilitate a number of key processes in drug discovery; and naturally our protein production capability feeds seamlessly into other HitGen functions/services, such as: 

  • X-ray Crystallography for SBDD / FBDD programs

  • Biochemical/Biophysical Assay Development

  • DNA Encoded Library based screening for hit identification


Our “crystallography grade” recombinant protein production can be available as stand-alone service or combine it with crystal structure determination.


HitGen Target Protein Production Overview

(In-house expressed over 110 target proteins, covering more than 20 different classes)

image.png

image.png

Questions?

For more details on how we can advance your innovative drug discovery projects
By submitting your information, you acknowledge having received, read and understood our Privacy Notice as made available above.

We use cookies to provide a better web experience.
By using our site, you acknowledge our use of cookies and please read our Cookie Notice for More information