Welcome to HitGen

Capabilities

One-stop Technology Platform to Accelerate Drug Discovery and Development

  1. Capabilities
  2. Pharmacodynamics

Pharmacodynamics

Pharmacodynamics is used to detect drug effectiveness in drug discovery and Investigational New Drug (IND) applications process.


In vitro pharmacology

Our in vitro pharmacology team has expertise across a variety of therapeutic areas, target types, assay formats and instrument, are expert in all kinds of enzymatic assays, binding assays and cellular assays.

Enzymatic assays

  • Light absorbance

  • Luminescence

  • Fluorescence intensity

  • HTRF and TR-FRET

Binding assays

  • Fluorescence polarization

  • ELISA

  • Alphascreen and AlphaLISA

Cellular assays

  • Cell viability, cell apoptosis, cell proliferation, cell cycle and cell death

  • Cell Migration Assay

  • Cell Invasion Assay

  • Cellular Uptake Assay

  • Cell co-culture Assay

  • Intracellular signaling: Calcium flux, Reporter Gene Assay

  • Protein expression: Western blotting , Immunostaining, Immunofluorescence, HTRF

  • Gene Knockdown by siRNA

  • Gene Knockout by CRISPR/Cas9

  • Stable cell line generation

  • FACS and high content imagine system


In vivo pharmacology

Our in vivo pharmacology team focuses on oncology and inflammation, is experienced with disease model establishment and validation for efficacy screening, in vivo pharmacology profiling, PK/PD and MOA studies.


Oncology

  • CDX models and SOC data

  • Syngeneic models with immune profile and SOC data



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